Johns Hopkins School of Medicine

Summer Internship Program 2019

Author: leitera

Learning in the Enteric Neurobiology Laboratory

The past two weeks of my internship have been really exciting. One of the things that I’ve been working on is removing the nodose ganglion from different strains of mice. This is located behind the vagus nerve, just on top of the skull. Learning to remove it was challenging as it is very small and breaks very easily. The best way to locate it to find the trachea of the mouse, and then locate the carotid artery. The Vagus nerve usually lies next to the artery. I was able to use a teaching microscope to learn how to do this. Once removing the nodose ganglion, I digested and cultured the cells. Next week, they will be grown in the organ chip. Experiments completed this week with the nodose ganglion have been used to prove that the cells can be successfully digested and cultured. The next challenge in this project will be to see how well they will be able to be grown in the organ-chip described in my previous post. 

using the teaching microscope with another undergrad in the lab. 

When I’m not working on this project, I’ve been learning about other lab members projects and assisting in those. This week, we did immunohistochemistry on stomach tissue. I’ve also been getting better at working with mice. I’ve learned to remove samples of RNA and protein from the guts of the mice, which will be needed later on this year for experiments in the enteric neurobiology lab. I recently was able to attend an entire day of seminars about cancer and the GI system. This was very interesting and relevant to my research as I study the connection between the gut and the brain. This was also super interesting to connect to what I’ve already learned about cancer on a cellular level in my classes at Dickinson.

Finally, I’ve been loving living in Baltimore. It’s a lot of fun living right next to the Johns Hopkins Campus. Recently, I had the opportunity to visit the National Aquarium located in the Inner Harbor area. I’m about halfway through my internship and have already learned so much, and can’t wait to see what else I learn this summer.

My First Two Weeks

My name is Ali Leiter and this summer I am working as an undergraduate researcher in the enteric neurobiology department in the gastroenterology unit at Johns Hopkins School of Medicine. This has been made possible by Dickinson’s Internship grant. I will be working on various projects, however my primary interest is creating a chip composed of cells that could replicate the gut-brain axis. This chip could potentially start to replace mouse-models that many laboratories use to conduct research. This summer, I will be working with mice to better understand the gut-brain axis. In the past two weeks, I’ve been making media to grow cells in, and making different solutions that preserve intestines and brains. Additionally, I’ve been completing a lot of research on the gut-brain axis in order to better understand the interactions between the two systems and the neural circuitry involved in their communication.

My lab is located in the Ross Research Building

My internship is located in Baltimore, Maryland. In my free time, I love going for runs around the city or hanging out with my roommates. This past weekend, I had the opportunity to explore the inner harbor area. There are so many good restaurants and interesting shops that I can’t wait to go back to. Transportation is super easy in the city too as Johns Hopkins provides a shuttle to all students, faculty and staff that can bring you all around Baltimore. I can’t wait to have the opportunity to see more places. Baltimore is very different from Carlisle– however, I’m excited to visit more places and get a better sense of the city.

I am so thankful for this opportunity, and can not wait to see what I am able to accomplish this summer!

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